Directly target and Locate Protein G
In an Instant
Fluro4 is a range of conjugative mixes that only dye the specially marked target microorganisms. After simply adding the mixture to the sample, and turning off the lights, Voila! Only the target particles will be illuminated .
Lighting the pathway to discovery
High contrast images of biomolecules
Using Fluro4 in conjunction with the GATOR4D to completely suppress the cellular auto fluorescence enables the capture of vivid, high contrast images of labelled cancer cells or biomolecules, with incredible prescision and massively reduced time requirements.
Delayed Luminescence Detection
Auto fluorescence is a ubiquitous phenomenon, numerous biological molecules fluoresce and the distribution and intensity of auto fluorescence is often so great that conventional FM has little utility. It is precisely the problem in detecting Cryptosporidium parvum and Mycobacterium tuberculosis in many samples. We completely alleviate this problem through our conjugative mixtures, which only illuminate the target molecules.
Searching for one person in a huge crowd at night, but that person has a light on their head
Delayed Luminescence Detection relies on special compounds that glow persistently after a short exposure to strong light. The bacteria or parasites are coated with the Fluro4 compounds and then viewed through the Gator4D which suppresses all light except that emitted by the coated bacteria. The effect is similar to searching for one person in a huge crowd at night, but that person has a light on their head.
We have a range of mixures to meet a variety of functions in the laboratory
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The product code for a luminophore is created using the following sequence of characters
ABCDxWN-nnnn
where –#nnn is the Batch CODE, # will range from A to Z and nnn from 1 to 999.
A = type of lanthanide
- E = Europium
- T = Terbium
- S = Samarium
- D = Dysprosium
- Pt = platinum
- X = other
B = ligand structure
- T = terphenyl
- B = 1,2 Dibenzyl benze (BHHBCB)
- C = Carbazole
- P = pyridine based
- D = DOTA
C = active binding group – the moiety that binds to target
- C = chlorosulfonate
- S = succinimide
- M = maleimide
D = conjugate status
- Dn = dendrimer of stage n
- Ab = antibody
- Aa = anti-antibody
- Ax = carrier of some other type of biomolecule
- X = not a conjugate
W = relative hydrophilic range – the ligand is dissolved in DMF at a concentration of 50 mg/ml and a maximum volume of 20 µL of this solution is added to 1ml of FEB solution.
- 0 = less than 100 µg/ml (hydrophobic)
- Range 1 to 8 corresponds to 100 µg/ml to 800 µg/ml (2 µL to 16 µL)
- 9 = water solubility of ligand exceeds 1 mg/ml
N = ligand status / chelated with target ion or not
- 1 = ligand is chelated with target ion
- 0 = ligand is pure and requires addition of ion
Example, BHHCT as pure ligand, has a chlorosulfonate binding group, is without added Eu3+ and was produced from the first batch A = E; B = T; C = C; D = X; W = 0; N = 0, batch code = A001 then BHHCT has product code ETCX00-A001